Tsingke tse005 2×t5 super pcr mix colony

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August undefined, 2024

Web2 O PCR primers OneTaq Master Mix 25 µl PCR Primer 200 nM H 2 O to 50 µl Note: If OneTaq Hot Start Quick-Load 2X Master Mix is used, reactions can be set up at room … WebJul 18, 2024 · Polymerase Chain Reaction (PCR) was performed in 12 μl reaction volumes containing 2 template DNA, 5 μl of 2 × T5 Super PCR Mix (PAGE; TSINGKE, Beijing, China), 1 μl of 10 μmol/μL primer pairs, and 4 μl of double distilled H 2 O. Target DNA segments were amplified with the following program, 95°C for 5 min, followed by 35 cycles of 95°C for 10 …

2×T5 Super PCR Mix（Colony） - Bio-Equip

WebProduct Details. TransTaq® -T PCR SuperMix is a ready-to-use mixture of TransTaq® -T DNA Polymerase, dNTPs and optimized buffer. The SuperMix is provided at 2× … Web4 Use 1 µl of reaction for colony PCR (T5 Taq Polymerase) 5 ... Concentration 2XT5 Super PCR Mix 5ul 1x 10uM Forward Primer 0.4ul 0.4uM 10uM Reverse Primer 0.4ul 0.4uM Template DNA 1ul <1ug highest euro to dollar

PCR SuperMix - Thermo Fisher Scientific

Web2) Pick 5 transformant colonies. 3) Grow each colony in respective antibiotic containing LB broth for 5-6 hours. 4) Took 500ul of culture and centrifuge. 5) Resuspend in 50ul of nuclease free ... Webtsingke-shop.oss-cn-beijing.aliyuncs.com http://www.tsingke.com.cn/products/details/12932 highest euro rate ever

2×T5SuperPCR_价格-品牌-详情介绍_丁香通 - biomart.cn

Does anyone have experience with colony PCR_Protocol and ...

WebApr 2, 2024 · GoldView TM Type I Nucleic Acid Electrophoresis Dye was purchased from Solarbio (Beijing, China), and 2×T5 Super PCR Mix was bought from TSINGKE (Hangzhou, … Web1.产品不推荐用于菌落和菌落PCR，如有需要推荐使用我公司专用PCR Mix（产品货号：TSE005）。 2.扩增脏模板时，可通过“稀释模板，增加循环数”或“减少模板量，扩大体 … highest ever boe base rateWebMar 7, 2024 · Pipette 8 µL of LB into PCR tubes and 5 mL (+ antibiotics) into glass culture tubes. Pick a colony off the agar plate and mix it into the LB in a PCR tube. Transfer 5 µL … highest euro note

"WebNov 1, 2024 · The reaction mixture was incubated for 60 min at 42°C and stored at −20°C until required. Single RT‐PCR. Single RT‐PCR was performed in a 25 µl reaction mixture containing 1·5 µl of cDNA, 22·5 µl of 1·1× T3 Super PCR Mix (Tsingke, Beijing, China) and 0·5 µl of forward and reverse primer each (20 µmol l −1 stock). " - Tsingke tse005 2×t5 super pcr mix colony

Tsingke tse005 2×t5 super pcr mix colony

Full article: A Rapid and Effective Colony PCR Procedure …

Web丁香通为您找到1条pcr非特异扩增产物多大信息，包括pcr非特异扩增产物多大报价行情，优质供应商，图片，品牌等最新信息，丁香通为买家提供用户服务，诚信保障等服务，批发采购pcr非特异扩增产物多大,上丁香通移动站。 Web我们联系：[email protected]。 2×T5 Super PCR Mix (PAGE) TSE006 产品组成： PCR体系与条件：目录号： 2×T5 Super PCR Mix (PAGE) 5×1.0 ml Component 2×T5 Super PCR Mix (PAGE) 10 μM Forward Primer 10 μM Reverse Primer Template DNA Nuclease-Free Water Final Concentration 1× 0.4 μM 0.4 μM <1 μg 推荐反应体系 ...

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Web2.5 TaqMan real-time RT-PCR assay. The novel PCR assay was developed by employing the 2×T5 Fast qPCR Mix (TSE301, TsingKe Biotech, China) on CFX384 Touch Real-Time PCR … Web2 ×T5 Super PCR Mix (Colony) 5. Initial denaturation. 98. 30s. 1 × . Forward primer . 0.2. Denaturation. 98. 10s. 29 × . Feverse primer . 0.2. Annealing. T m-5. 30s. Template. 2. …

http://www.transgenbiotech.com/data/upload/image/20241231/1609386732127122.pdf WebJan 6, 2024 · Then, qRT-PCR was carried out on the CFX96 instrument (Bio-Rad, USA) with Ssofast EvaGreen Supermix Kit in a 20 µl reaction volume containing 0.8 µl of each primer, …

WebMoreover, compared to a commercially available kit (2 × T5 Direct PCR kit) (Tsingke, Beijing, China), the electrophoresis results of this colony PCR was also satisfactory with specific … WebMar 5, 2024 · Then, colony PCR was used to identify the bacterial strain. Briefly, about 2 μL of bacteria were picked into a 50 μL 2 × T5 Super PCR Mix (Colony) system (TSINGKE Biological Technology, Beijing, China) and then amplified and sequenced using primers 27F (5′-AGAGTTTGATCCTGGCTCAG-3′) and 1492R (5′-GGTTACCTTGTTA CGACTT-3′).

http://tsingke-shop.oss-cn-beijing.aliyuncs.com/TSE008-2%C3%97T5%20Super%20PCR%20Mix%28Basic%29%E8%AF%B4%E6%98%8E%E4%B9%A6V1.1.3.pdf

Webmy method: 50 ul h20, pic with a yellow tip a E. coli colony, mix with the water, them heat in the PCR machine at 98ºC, for 10 min, them leave at 4ºc for 5 min. Centrifuge 13000g /10 min, use 2 ... highest ever cpu clockWebFeatures of Platinum SuperFi II PCR Master Mix include: • Exceptional >300X Taq fidelity. • Universal primer annealing at 60°C. • Superior specificity, sensitivity, and yields. • Robust amplification of difficult-to-amplify targets (e.g., those with suboptimal purity, ˃65% GC content, long PCR requirement) highest euro to dollar everWebqRT-PCR Analysis For qRT-PCR analysis, we extracted RNA using the RNAprep Pure Plant Kit (TIANGEN, Beijing, ... The 2 × T5 Super PCR Mix (Colony) was used. ... highest ever dow jones closehttp://tsingke-shop.oss-cn-beijing.aliyuncs.com/TSE005-2%C3%97T5%20Super%20PCR%20Mix%20%28Colony%29%E8%AF%B4%E6%98%8E%E4%B9%A6V1.1.2.pdf how get gmail on iphoneWebFeb 18, 2024 · Prime STAR Max DNA polymerase was bought from Takara (Shanghai, China), T5 super PCR Mix (Colony) DNA polymerase, ... Trelidf™ Prestained Protein … highest ever bitcoin priceWeb2×T5 Super PCR Mix (Colony) TSE005 产品说明：目录号： T5 DNA 聚合酶是新型DNA 聚合酶，由经过改造的Taq DNA 聚合酶融合Sso7d DNA结合域而得来，具有极高的扩增速度 … how get gas smell out of clothesWebJan 1, 2013 · A single E.coli colony can be used for simultaneous PCR amplification and overnight growth. First, disperse some of the scraped colony into the PCR master mix. Disperse the remainder into a tube containing 1–2 ml of LB medium plus the appropriate antibiotic and grow overnight at 37 °C with shaking. how get gum out of clothes