Halolysin
WebAug 3, 2024 · Halolysin SptA is the major extracellular protease of Natrinema sp. J7-2, and its production is growth-phase dependent. The growth phase-dependent production of halolysin has been described in some haloarchaea such as Ncc. occultus (Studdert et al., 1997), Nab. asiatica (Kamekura and Seno, 1990), and Nab. magadii (Paggi et al., 2010). WebJun 11, 2024 · A gene encoding a halophilic serine proteinase, halolysin R4, from a halophilic archaeon Haloferax mediterranei strain R4 was cloned, its nucleotide sequence determined, and expressed in Haloferax volcanii WFD11. The deduced amino-acid sequence (403 aa in length) showed the highest s:[milarity to halolysin 172P1, produced …
Halolysin
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WebJun 14, 2008 · The Hfx. volcanii synthesized protease was active in high salt, high pH and high DMSO. This study provides the first molecular characterization of a halolysin-like protease from alkaliphilic haloarchaea and is the first description of a recombinant system that facilitates high-level secretion of a haloarchaeal protease. WebGenerally, HlyA from the extremely halophilic archaeon Hcc. salifodinae is an alkaliphilic and low salt-adapted halolysin with high activity, thus representing an attractive candidate for various industrial uses.
Webjournals.asm.org Webhemolysin: [noun] a substance that causes the dissolution of red blood cells.
WebAug 3, 2024 · Halolysin SptA, the major extracellular protease of Natrinema sp. J7-2, is capable of intracellular self-activation to affect haloarchaeal growth. Here, we report that … Webhe·mol·y·sin. (hĭ-mŏl′ĭ-sĭn, hē′mə-lī′-) n. An agent or substance, such as an antibody or a bacterial toxin, that causes the destruction of red blood cells, thereby liberating …
WebHalolysin SptA from haloarchaeon Natrinema sp. J7 consists of a subtilisin-like catalytic domain and a C-terminal extension (CTE) containing two cysteine residues. In this report, we have investigated the function of the CTE using recombinant enzymes expressed in Haloferax volcanii WFD11. Deletion of the CTE greatly reduced but did not abolish ...
WebHalolysin precursors contain a twin arginine signal pep-tide, a propeptide, a subtilase catalytic domain and a C-ter-minal extension (CTE). During or after the trans-membrane … tes alergi di bekasiWebJan 15, 2024 · Sequence (a) and crystal structure (b and c) of a polypeptide chain fragment from the surface layer protein tetrabrachion from Staphylothermus marinus showing the right-handed parallel RHCC tetramer. In (a) the sequence positions are labelled from a to k with the hydrophobic core positions highlighted in red. (b) Axial view from the N-terminus ... tes alergi adalahWebFeb 12, 2024 · Halolysin has a long C-terminal extension of approximately 120 amino acids which has not been found in other extracellular subtilisin type serine proteases. The … tes alergi dimanaWebHalolysin proteins had higher average of obligatory halophilic microorganisms that have negatively charged amino acids comparing to other been adapted to optimal growth under conditions of proteins. Asp and Glu, two negatively charged extremely high salinity. They contain a amino acids with average of 0.091 and 0.053, correspondingly high ... tes alergi di jakartaWebHalolysin SptA from haloarchaeon Natrinema sp. J7 consists of a subtilisin-like catalytic domain and a C-terminal extension (CTE) containing two cysteine residues. In this report, we have investigated the function of the CTE using recombinant enzymes expressed in Haloferax volcanii WFD11. Deletion of the CTE greatly reduced but did not abolish ... tesa leroy merlinWebFeb 12, 2024 · Importance: This study shows that halolysin R4 from Haloferax mediterranei provides its host antagonistic and defensive activities against other haloarchaea, which expands our knowledge on the traditional function of haloarchaeal extracellular proteases. Haloarchaeal extracellular serine proteases have been previously discussed as growth … tes alergi makananWebFeb 1, 2015 · In the present study, we showed that the Tat-dependent halolysin SptA matures autocatalytically and that premature activation of SptA in the heterologous host … tesalia arx 2017