Glycoworks hilic elution plate
WebJan 11, 2024 · Glycosylation is a critical quality attribute of monoclonal antibody (mAb) therapeutics. Hydrophilic interaction liquid chromatography-mass spectrometry (HILIC-MS) is an invaluable technology for the characterization of protein glycosylation. HILIC/MS-based glycan analysis relies on the library search using Glucose Units (GU) and accurate mass … WebThe Glycoworks® RapiFluor-MS™ Clean-up Module contained a Glycoworks® HILIC μ-Elution Plate, Glycoworks® SPE elution buffer (200 mM ammonium acetate, 5% ACN), and Glycoworks® Sample Diluent (DMF). The Glycoworks® RapiFluor-MS™ Sample Collection Module contained sample collection tubes, a waste tray, and a collection tray. …
Glycoworks hilic elution plate
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WebThe GlycoWorks HILIC uElution Plate is ideal for removing contaminants like salts and detergents from hydrophilic analytes, such as carbohydrates, prior to mass spectrometry … WebTo evaluate this step, elution from the GlycoWorks HILIC sorbent was studied in detail. 2-AB labeled glycans were loaded onto a 96-well HILIC µElution Plate according to the protocol provided in the GlycoWorks High-throughput Sample Preparation Kit Care and Use Manual. 13 Various eluents were then employed for elution
Webkits/186008939-glycoworks-rapid-deglycosylation-kit---1-x-24.html> ) was diluted with 220 µL of water. Deglycosylation was begun by dispensing the 10 µL of glycoprotein at 1.2 mg/mL in a 96 well plate, followed by the additions of 10 µL of 3% (w/v) RapiGest with 2 µg/µL DTT denaturing buffer. After heat denaturation at 90 °C WebGlycoWorks HILIC SPE 96-well µElution Plates (30 pmol of glycans processed). Percent recoveries as a function of experimentally determined glucose units (GU) are shown for various elution conditions. Values are based on the average of three replicate analyses. Trisialylated 25 mM NH 4HCO 3 100 mM NH 4OAc 25 mM NH 4OAc 10 mM NH 4OAc ...
WebThe released N-glycans were recovered using a HILIC-SPE Elution plate (Waters) after washing (90% ACN), elution (1 mM Ammonium Tris Citrate in 10% ACN) and evaporation to dryness. Labelling of the released N-glycans was performed by incubation with the 2-AB dye (dissolved in 30% acetic acid in DMSO) at 65 C for 3 h in the dark. Then, excess WebApr 4, 2024 · N-Glycan analysis is routinely performed for biotherapeutic protein characterization. A recently introduced N-glycan analysis kit using RapiFluor-MS (RFMS) labeling provides time savings over reductive amination labeling methods while also providing enhanced fluorescence (FLR) and mass spectrometry (MS) responses. This …
WebMar 5, 2024 · The Glycoworks ® RapiFluor-MSTM Clean-up Module contained a Glycoworks ® HILIC μ-Elution Plate, Glycoworks ® SPE elution buffer (200 mM ammonium acetate, 5% ... The derivatized N-linked glycans were purified using a HILIC μ-Elution plate. The medium was first washed with three 200 μL aliquots of water, which …
WebSteel Flitch Plates, Flitch Beams. Aluminum Flitch Plates. Stainless Flitch Plates. Flitch Beams Any Size or Length flitch plate or Flitch Beam, call to confirm email for faster … race car bedding kidsracecar bed jankWebThis application brief demonstrates the benefits of using HILIC as an ideal separation mode for glycans as well as the robustness of HILIC in an SPE format. shockwave overlayWebIn this work, 2-AB labeled glycans were loaded onto a 96-well HILIC μElution plate according to the protocol provided in the GlycoWorks High-throughput Sample … shockwave out of contextWebWaters Glycoworks TMRapiFluor-MS kit reagents and pro-tocols (Waters, Milford, MA, USA). rhEPO preparations were ... HILIC Elution plate, with elution in 3×30 l 200mM ammonium formate in 5% (v/v ... race car bed redWebRe-imagine a streamlined, fast, and highly sensitive way to analyze released N-glycans. With just 3 easy steps in as little as 30 minutes, the GlycoWorks RapiFluor-MS N … shockwave overlay downloadWebGlycoWorks HILIC µElution Plate or 1 cc Cartridge GlycoWorks Reagent Kit* (RapiGest™ SF, IAM, and DTT) Denaturation Reduction Alkylation Deglycosylation with PNGase F ... 96-Well Elution Plate Single-Use 1cc Cartridge A B C Cartridges (single-use) ( p/n 186007080 ) is shown in Figure 2B. Visual inspection shockwave owner