Fk2 antibody

WebMar 20, 2024 · Ubiquitin staining was performed using anti-ubiquitin (FK2) antibody. The blue (Hoescht 33342) but not red bacteria are L. dumoffii. Ubiquitin was commonly associated with LpCVs (arrows), but was ... WebFeb 21, 2014 · The third method utilized the anti-ubiquitin antibody FK2. In the initial studies, the FK2 method was the most efficient method of isolating endogenously ubiquitinated protein complexes and recognizing monoubiquitinated and …

Anti-Ubiquitin Mouse mAb (FK2) ST1200 - Merck Millipore

WebThe FK2 antibody works great in both human and rat samples (I haven't tried mouse) by western blot and immunofluorescence. This antibody should recognize the ubiquitin modifications (both mono... WebJan 7, 2024 · product name : Mono- and polyubiquitinylated conjugates monoclonal antibody (FK2) catalog : BML-PW8810-0100 quantity : 100 µg clonality : monoclonal host : mouse conjugate : nonconjugated clone name : FK2 reactivity : southern flounder, Saccharomycetales, human, mouse, rat, fruit fly application : oodle epic games https://redwagonbaby.com

Anti-Multi Ubiquitin mAb(Monoclonal, FK2) Antibody MBL …

WebJan 7, 2024 · antibody product name : Polyubiquitinylated conjugates monoclonal antibody (FK1) catalog : BML-PW8805-0500 quantity : 500 µg clonality : monoclonal host : mouse conjugate : nonconjugated clone name : FK1 reactivity : spiny dogfish, human, mouse, rat, fruit fly application : western blot, ELISA, immunohistochemistry, immunocytochemistry, … WebUbiquitin modification of p62 was detected using an antibody recognizing mono- and poly-ubiquitin (FK2) (lower panels). The EGFP–TRIM proteins were detected using an anti-GFP antibody. * indicates mono-ubiquitylated p62 and a high-molecular-mass p62. WebSep 10, 2016 · Scheme of the ELISA assay. (a) In the “forward” approach, microwell plates are coated with a polyclonal anti-EGFR intra, which captures the receptor from the lysate (1).Detection of Ub-EGFR or total EGFR (to normalize) is performed with primary monoclonal antibodies directed against Ub (FK2, 1) or EGFR extra (2), respectively, … oodle decompression library 2.9.5

The dynamics of histone H2A ubiquitination in HeLa cells

Category:Polyubiquitinylated conjugates monoclonal antibody (FK1) Enzo …

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Fk2 antibody

Fk2 Monoclonal Antibody Biomol GmbH Bioz

WebWestern blots were developed using PVDF membranes and A-106 anti-ubiquitin primary antibody at 0.5 µg/ml followed by HRP-labeled anti-mouse (R&D Systems # HAF007) … WebThis Anti-Ubiquitin Mouse mAb (FK2) is validated for use in ELISA, Immunoblotting, Immunoprecipitation, Immunofluorescence for the …

Fk2 antibody

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WebNational Center for Biotechnology Information WebMar 25, 2016 · We previously developed an FK2 antibody, which specifically recognizes poly-Ub moie … Polyubiquitination plays key roles in proteasome-dependent and independent cellular events, whereas monoubiquitination is involved in gene expression, DNA repair, protein-protein interaction, and protein trafficking.

WebClone FK2 Antibody Products. Clone FK2 is reported to be a monoclonal antibody specific to the human gene, UBB. Indicated applications for antibody clone FK2 include Western … WebImmunocytochemistry (ICC) analysis of ubiquitinylated protein conjugates using FITC labeled mono- and polyubiquitinylated conjugates mAb (UBCJ2), (Prod. No. ENZ …

WebFeb 11, 2024 · Anti-Multi Ubiquitin mAb(Monoclonal, FK2 from MBL.Clone FK2 has been reported to recognize K29-, K48-, K63-linked poly ubiquitinylated and mono … WebSep 13, 2024 · The FK2 monoclonal ubiquitin antibody is a frequently used and widely-cited ubiquitin antibody that detects both mono- and polyubiquitin, but not free ubiquitin. …

WebThe Tb-anti-ubiquitin-FK2 antibody preferentially binds to mono- or poly-ubiquitinated proteins over free ubiquitin. This antibody can be used in combination with purified GFP …

WebRibosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is … oodle dictionaryWebJun 30, 2024 · For the analysis of human cells, OtUBD pulldowns performed better than FK2 antibody immunoprecipitations in our hands, both in terms of overall efficiency and repeatability. Inconsistency in the performance of antibodies from different batches has been a widely recognized issue . Recombinant OtUBD, by contrast, appeared to be … iowacarematchweb.com/csp/idhs/scrlogon.cspoodle finance customer serviceWebAug 17, 2011 · The FK1 and FK2 monoclonal antibodies are used to differentiate between monoubiquitination and polyubiquitination, since FK2 detects both monoubiquitinated and polyubiquitinated proteins, while FK1 recognizes only polyubiquitinated proteins (Fujimuro et al., 1994). Therefore, to assess whether the ubiquitination of PSD-95 reflects ... oodle free advertisingWebUbiquitin Antibody - Mouse Monoclonal antibody to Ubiquitin, FK2. Species Independent. Validated for use in Western Blot, IHC, ICC/IF, IP, and ELISA. oodle finance chatWebClone FK2 is the most widely used ubiquitin antibody. This clone reacts with mono- and polyubiquitinated conjugates, but does not react with free ubiquitin. 1, 2 WARNING This product is not for human or veterinary use. Certificates of Analysis & Batch Specific Data Get Batch-Specific Data and Documents by Batch Number oodlegrandjunctioncoWebGeneral description. Mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with myeloma cells. Recognizes K 29 -, K 48 -, and K 63 -linked polyubiquitinylated and monoubiquitinylated proteins. Recognizes K 29 -, K 48 -, and K 63 -linked polyubiquitinylated and monoubiquitinylated ... oodle harley davidson