We first employed the immunofluorescence staining assay to validate the overexpression of ALP on the membranes of HeLa cells (Supplementary Fig. 12)37. On incubation with P-CyPt, ALP-positive HeLa cells displayed distinct mCy’s FL around the cell membranes, which intensified with the concentration and … See more P-CyPt is designed to (1) offer activatable near-infrared (NIR) FL and PA signals for bimodality imaging through ALP-mediated in situ self-assembly and (2) release CDDP for on … See more We first quantified the amount of Pt(II) in HeLa cells by ICP-OES, which showed a large amount of Pt (24.60 ± 3.5 fmol/cell) in P-CyPt-treated … See more P-CyPt was synthesized according to the procedure illustrated in Supplementary Methods. We first monitored the ALP-triggered dephosphorylation and self-assembly of P-CyPt … See more After intravenous (i.v.) injection of P-CyPt into subcutaneous (s.c.) HeLa tumor-bearing mice, tumors displayed strong FL at 1 h, which was maintained for over 4 h (Fig. 4a). In contrast, though preformed fluorescent PtIVNPs … See more WebJul 24, 2024 · Cy-COOH (36.0 mg, 0.044 mmol) and 1-ethyl-3- (3-dimethylaminopropyl)carbodiimide hydrochloride (EDCI) (20 mg, 0.1 mmol) were dissolved in 1 mL of dry dimethylformamide (DMF) and it was stirred for 15 min, followed by the addition of 1 mL dry DMF solution containing N -hydroxysuccinimide (NHS) (5.1 mg, …
Biosensor of alkaline phosphatase based on non-fluorescent
WebMay 1, 1994 · Europe PMC is an archive of life sciences journal literature. Search life-sciences literature (39,392,389 articles, preprints and more) WebOct 11, 2024 · Firstly, a series of cyanine-structural small molecule with different-charged substituent group have been screened based on their mitochondria targeting capability. Furthermore, the... fishing rewards wow
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WebJun 11, 2024 · I Intermolecular C III Cc Cf1 Cf2 IV V Cy COOH Figure 1 Divisions of the primary structure of LMW glutenin subunits according to Wieser9, cysteine designation according to Kohler et al.10, and proposed disulphide structure. ¨ Disulphide bonds play a key role in determining the structure of gluten and are essential for the unique … WebCy (CH2CH2O)n CH2CH2 COOH 5 O NH Product Specifications: Composition: Cy5 PEG COOH. Ex/Em: 650/670 nm Appearance: Blue/dark blue solid, semi-solid depends on molecular weight. Solubility: Soluble in water, chloroform, DMSO. Reactive group: -COOH, Handling and Use: Cy5 PEG acid should always be kept in low temperature in dry condition. http://www.duofluor.com/product/cy7-cooh/ cancel credit card to stop recurring charges